Spin-labeled sulfonyl fluorides as active site probes of protease structure. I. Comparison of the active site environments in alpha-chymotrypsin and trypsin.
نویسندگان
چکیده
& R R = spin label incorporated in cu-chymotrypsin and trypsin (1). The spectral results also show the power of applying such a method to structural comparison of active sites of new proteases whose threedimensional structures are not yet known. We present here the detailed syntheses and purifications of these spin labels and some of the specific problems encountered in preparing the inhibited sulfonylated enzyme derivatives.
منابع مشابه
Spin-labeled Sulfonyl Fluorides as Active Site Probes of Protease Structure
a-Chymotrypsin and trypsin were spin-labeled with 14 ortho-, meta-, and para-substituted phenylsulfonyl fluorides. These analogues of tosylfluoride were substituted with either pyrrolidinyl, pyrrolinyl, or piperidinyl nitroxide moieties. A complete description of their synthesis and the enzyme inhibition studies is presented in the following paper (WONG, S. S., QUIGGLE, K., TRIPLETT, C., AND BE...
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 249 6 شماره
صفحات -
تاریخ انتشار 1974